THE TOXICOLOGICAL EFFECT OF CYCLOPHOSPHAMIDE ON ACETYLCHOLINESTERASE ACTIVITY

被引：16

作者：

ALJAFARI, AA

机构：

[1] Department of Biochemistry, College of Science, King Saud University, Riyadh

来源：

TOXICOLOGY LETTERS | 1993年 / 66卷 / 02期

关键词：

ANTICANCER DRUG; KINETIC STUDY; INHIBITION;

D O I：

10.1016/0378-4274(93)90087-E

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

Cyclophosphamide (CP; 2.5-10.0 mM) reversibly inhibits acetylcholinesterase (AChE) activity of chicken brain (58-84%) in a concentration-dependent manner, the IC50 (the concentration causing 50% inhibition) being about 2.0 mM. Lineweaver-Burk plots indicated that the nature of this inhibition is mixed; intercept and slope replots are straight lines, showing that the inhibition is complete rather than partial. The value of K(m) for AChE is found to be 108 muM, while values of K(I) and K(i) are found to be 1.5 and 5.1 mM, respectively. K(i) is greater than K(I) indicating that uncompetitive inhibition is predominant over noncompetitive inhibition.

引用

页码：125 / 131

页数：7

共 11 条

[1] LOCAL-ANESTHETICS - A NEW CLASS OF PARTIAL INHIBITORS OF MITOCHONDRIAL ATPASE [J].