ALKALINE PHOSPHATASE-MODIFIED CARBON-FIBER DISK MICROELECTRODE FOR THE DETERMINATION OF 4-AMINOPHENYL PHOSPHATE

被引：12

作者：

PARIENTE, F ^{[1
]}

HERNANDEZ, L ^{[1
]}

ABRUNA, HD ^{[1
]}

LORENZO, E ^{[1
]}

机构：

[1] UNIV AUTONOMA MADRID,FAC CIENCIAS,DEPT QUIM ANALIT & ANAL INSTRUMENTAL,E-28049 MADRID,SPAIN

来源：

ANALYTICA CHIMICA ACTA | 1993年 / 273卷 / 1-2期

关键词：

AMPEROMETRY; BIOSENSORS; ENZYMATIC METHODS; ALKALINE PHOSPHATASE-MODIFIED ELECTRODE; AMINOPHENYL PHOSPHATE; CARBON FIBER DISK MICROELECTRODE; ENZYME ELECTRODES;

D O I：

10.1016/0003-2670(93)80157-G

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The construction and response of an alkaline phosphatase-modified carbon fibre disc microelectrode and its use as an amperometric sensor for the determination of 4-aminophenyl phosphate are described. The enzyme was immobilized on the electrode surface using glutaraldehyde and bovine serum albumin as cross-linking agents. The product of the enzymatic reaction, 4-aminophenol, was determined by oxidation at +0.3 V vs. a sodium chloride saturated calomel electrode, yielding a steady-state current directly related to the bulk concentration of the substrate. The response time of this microbiosensor was typically less than 3 s and 4-aminophenyl phosphate could be determined in the range 5.0 x 10(-7) -5.0 x 10(-3) M.

引用

页码：187 / 193

页数：7

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