RAPID ESTIMATION OF GENETIC RELATEDNESS AMONG HETEROGENEOUS POPULATIONS OF ALFALFA BY RANDOM AMPLIFICATION OF BULKED GENOMIC DNA SAMPLES

被引：153

作者：

YU, KF ^{[1
]}

PAULS, KP ^{[1
]}

机构：

[1] UNIV GUELPH,DEPT CROP SCI,GUELPH N1G 2W1,ONTARIO,CANADA

来源：

THEORETICAL AND APPLIED GENETICS | 1993年 / 86卷 / 06期

关键词：

POLYMERASE CHAIN REACTION; RANDOM PRIMERS; GENETIC DISTANCE; RANDOM AMPLIFIED POLYMORPHIC DNA; MEDICAGO-SATIVA;

D O I：

10.1007/BF00222671

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

A procedure which involves the use of RAPD markers, obtained from bulked genomic DNA samples, to estimate genetic relatedness among heterogeneous populations is demonstrated in this study. Bulked samples of genomic DNA from several alfalfa plants per population were used as templates in polymerase chain reactions with different random primers to produce RAPD patterns. The results show that the RAPD patterns can be used to determine genetic distances among heterogeneous populations and cultivars which correspond to their known relatedness. The results also indicate that, by using ten primers with bulked DNA samples from ten individuals, 18-72 populations or cultivars can be distinguished from each other on the basis of at least one unique RAPD marker. We anticipate that DNA bulking and methods for comparing RAPD patterns will be very useful for identifying cultivars, for studying phylogenetic relationships among heterogeneous populations and for selecting parents to maximize heterosis in crosses.

引用

页码：788 / 794

页数：7

共 21 条

[1] RESTRICTION FRAGMENT LENGTH POLYMORPHISMS AS GENETIC-MARKERS IN SOYBEAN, GLYCINE-MAX (L) MERRILL
APUYA, NR
FRAZIER, BL
KEIM, P
ROTH, FJ
LARK, KG
[J]. THEORETICAL AND APPLIED GENETICS, 1988, 75 (06) : 889 - 901
[2] DNA AMPLIFICATION FINGERPRINTING USING VERY SHORT ARBITRARY OLIGONUCLEOTIDE PRIMERS
CAETANOANOLLES, G
BASSAM, BJ
GRESSHOFF, PM
[J]. BIO-TECHNOLOGY, 1991, 9 (06): : 553 - 557
[3] RAPID IDENTIFICATION OF GENETIC-VARIATION AND PATHOTYPE OF LEPTOSPHAERIA-MACULANS BY RANDOM AMPLIFIED POLYMORPHIC DNA ASSAY
GOODWIN, PH
ANNIS, SL
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1991, 57 (09) : 2482 - 2486
[4] Hallauer AR, 1988, QUANTITATIVE GENETIC
[5] USE OF SINGLE-PRIMER DNA AMPLIFICATIONS IN GENETIC-STUDIES OF PEANUT (ARACHIS-HYPOGAEA L)
HALWARD, T
STALKER, T
LARUE, E
KOCHERT, G
[J]. PLANT MOLECULAR BIOLOGY, 1992, 18 (02) : 315 - 325
[6] DNA FINGERPRINTING - A TOOL FOR DETERMINING GENETIC DISTANCES BETWEEN STRAINS OF POULTRY
KUHNLEIN, U
DAWE, Y
ZADWORNY, D
GAVORA, JS
[J]. THEORETICAL AND APPLIED GENETICS, 1989, 77 (05) : 669 - 672
[7] RAPID IDENTIFICATION OF MARKERS LINKED TO A PSEUDOMONAS RESISTANCE GENE IN TOMATO BY USING RANDOM PRIMERS AND NEAR-ISOGENIC LINES
MARTIN, GB
WILLIAMS, JGK
TANKSLEY, SD
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (06) : 2336 - 2340
[8] DIVERSITY AND RELATIONSHIPS AMONG UNITED-STATES MAIZE INBREDS REVEALED BY RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISMS
MELCHINGER, AE
MESSMER, MM
LEE, M
WOODMAN, WL
LAMKEY, KR
[J]. CROP SCIENCE, 1991, 31 (03) : 669 - 678
[9] IDENTIFICATION OF MARKERS LINKED TO DISEASE-RESISTANCE GENES BY BULKED SEGREGANT ANALYSIS - A RAPID METHOD TO DETECT MARKERS IN SPECIFIC GENOMIC REGIONS BY USING SEGREGATING POPULATIONS
MICHELMORE, RW
PARAN, I
KESSELI, RV
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (21) : 9828 - 9832
[10] Nei M., 1987, MOL EVOLUTIONARY GEN, DOI [DOI 10.7312/NEI-92038, 10.7312/nei-92038]

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