METABOLIC LABELING OF ENTAMOEBA-HISTOLYTICA ANTIGENS - CHARACTERIZATION OF A 28-KDA MAJOR INTRACELLULAR ANTIGEN

被引：14

作者：

BHATTACHARYA, A ^{[1
]}

BHATTACHARYA, S ^{[1
]}

SHARMA, MP ^{[1
]}

DIAMOND, LS ^{[1
]}

机构：

[1] NIAID, PARASIT DIS LAB, BETHESDA, MD 20892 USA

来源：

EXPERIMENTAL PARASITOLOGY | 1990年 / 70卷 / 03期

关键词：

Antigenic analysis; Entamoeba histolytica; Metabolic labeling; Secretory proteins; Triton X-114 detergent phase extraction;

D O I：

10.1016/0014-4894(90)90107-N

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

The in vivo incorporation of radiolabeled amino acids into antigens of Entamoeba histolytica, HM-1,IMSS, is reported. Immunoprecipitation with sera from patients with invasive amebiasis revealed a 28-kDa antigen present in whole cell lysates of E. histolytica. This antigen was of cytoplasmic origin, as indicated by cell fractionation and Triton X-114 detergent-phase separation. Immunoprecipitation, using sera from patients with invasive amebiasis and symptomless cyst passers, revealed the 28-kDa antigen as the major antigen recognized by the sera tested. Immunoprecipitation analysis using radiolabeled-released proteins instead of whole cell lysates showed a number of bands, including the 28-kDa antigen. The data suggest that the 28-kDa antigen is of cytoplasmic origin or is released from the cytoplasmic compartment. © 1990.

引用

页码：255 / 263

页数：9

共 23 条

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