METABOLIC LABELING OF ENTAMOEBA-HISTOLYTICA ANTIGENS - CHARACTERIZATION OF A 28-KDA MAJOR INTRACELLULAR ANTIGEN

被引:14
作者
BHATTACHARYA, A [1 ]
BHATTACHARYA, S [1 ]
SHARMA, MP [1 ]
DIAMOND, LS [1 ]
机构
[1] NIAID, PARASIT DIS LAB, BETHESDA, MD 20892 USA
关键词
Antigenic analysis; Entamoeba histolytica; Metabolic labeling; Secretory proteins; Triton X-114 detergent phase extraction;
D O I
10.1016/0014-4894(90)90107-N
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
The in vivo incorporation of radiolabeled amino acids into antigens of Entamoeba histolytica, HM-1,IMSS, is reported. Immunoprecipitation with sera from patients with invasive amebiasis revealed a 28-kDa antigen present in whole cell lysates of E. histolytica. This antigen was of cytoplasmic origin, as indicated by cell fractionation and Triton X-114 detergent-phase separation. Immunoprecipitation, using sera from patients with invasive amebiasis and symptomless cyst passers, revealed the 28-kDa antigen as the major antigen recognized by the sera tested. Immunoprecipitation analysis using radiolabeled-released proteins instead of whole cell lysates showed a number of bands, including the 28-kDa antigen. The data suggest that the 28-kDa antigen is of cytoplasmic origin or is released from the cytoplasmic compartment. © 1990.
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页码:255 / 263
页数:9
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