A NEW APPROACH TO THE NEWBORN SCREENING FOR HYPERPHENYLALANINEMIAS - USE OF L-PHENYLALANINE DEHYDROGENASE AND MICROTITER PLATES

被引：26

作者：

WENDEL, U

KOPPELKAMM, M

HUMMEL, W

SANDER, J

LANGENBECK, U

机构：

[1] STAATL MED UNTERSUCHUNGSAMT,HANNOVER,GERMANY

[2] HEINRICH HEINE UNIV,INST ENZYMTECHNOL,JULICH,GERMANY

[3] UNIV FRANKFURT KLINIKUM,INST HUMAN GENET,W-6000 FRANKFURT 70,GERMANY

来源：

CLINICA CHIMICA ACTA | 1990年 / 192卷 / 03期

关键词：

Enzymatic analysis; Hyperphenylalaninemia; Newborn screening; Phenylketonuria;

D O I：

10.1016/0009-8981(90)90218-H

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

We adapted the recently described colorimetric method for the specific determination of phenylalanine to a microplate assay using a NAD(H)-dependent L-phenylalanine dehydrogenase. With respect to sensitivity, analytical recovery and interrun imprecision this method for measuring phenylalanine in eluates of paperdried blood spots is suitable for routine newborn screening for hyperphenylalaninemias. In contrast to the microbiological Guthrie assay, with the enzymatic method quantitative data may be obtained on the same day, also in the blood of newborns on antibiotic treatment. © 1990.

引用

页码：165 / 170

页数：6

共 10 条

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AMBROSE JA, 1973, HEALTH LAB SCI, V10, P180

[2] COMPARATIVE COLUMN CHROMATOGRAPHIC ESTIMATIONS OF PHENYLALANINE IN PLASMA, WHOLE-BLOOD, NATIVE AND PAPER-DRIED CAPILLARY BLOOD OF HEALTHY-CHILDREN AND ADULTS, AND PATIENTS WITH HYPERPHENYLALANINEMIA [J].