ALTERNATIVE SPLICING OF THE HOX-2.2 HOMEOBOX GENE IN HUMAN HEMATOPOIETIC-CELLS AND MURINE EMBRYONIC AND ADULT TISSUES

The HOX 2.2 homeobox gene is expressed in human hematopoietic cell lines with erythroid features (W.-F. Shen, et al, Proc. Natl. Acad. Sci. 86, 8536-8540, 1989). Both human and murine Hox 2.2 genes contain a single 1 kb intron which interrupts the sequence encoding the proposed homeobox protein. Four human erythroleukemia cell lines express the spliced, homeobox-coding transcript as the major form of message, and variable low amounts of unspliced HOX 2.2 mRNAs. Murine embryonic tissues and adult kidney and uterus contain approximately equal amounts of transcripts containing this intron and mRNAs from which the intron has been excised. The spliced transcript encodes a 224 amino acid homeobox protein, while the unspliced transcript would potentially encode a 140 residue protein containing the same N-terminal sequence but lacking the homeodomain.