PROSPECTIVE SERIAL STUDY OF VIRAL CHANGE IN THE CERVIX AND CORRELATION WITH HUMAN PAPILLOMAVIRUS GENOME STATUS

被引:5
作者
KITCHENER, HC
NEILSON, L
BURNETT, RA
YOUNG, L
MACNAB, JCM
机构
[1] UNIV GLASGOW,MRC,VIROL UNIT,GLASGOW G12 8QQ,SCOTLAND
[2] WESTERN INFIRM & ASSOCIATED HOSP,DEPT PATHOL,GLASGOW G11 6NT,SCOTLAND
[3] UNIV BIRMINGHAM,DEPT CANC STUDIES,BIRMINGHAM B15 2TT,W MIDLANDS,ENGLAND
来源
BRITISH JOURNAL OF OBSTETRICS AND GYNAECOLOGY | 1991年 / 98卷 / 10期
关键词
D O I
10.1111/j.1471-0528.1991.tb15344.x
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
Objective - To observe in serial fashion the histological changes of human papilloma virus (HPV) infection of the cervix and to correlate these with the detection of HPV genomes. Design - A prospective longitudinal study to perform colposcopy and at 0, 9 and 18 months to obtain a smear, a biopsy for histology and HPV-16 genome detection by DNA/DNA hybridization. Setting - Glasgow Family Planning Centre. Subjects - Eighty-two women recruited on the basis of a routine smear showing viral change without dyskaryosis. Interventions - Women found to have CIN 3 were treated with laser. Main outcome measures - Cytology and cervical biopsy results and HPV-16 DNA detection by Southern blotting obtained serially from individual subjects, Results - Of 82 women recruited, 10% were positive for HPV-16 detection by Southern blotting. Detection of HPV-16 genomes did not correlate with either CIN or viral infection assessed histologically and serial hybridizations in a given individual showed fluctuation in HPV-16 detection. Polymerase chain reaction in a subset of samples revealed 46% positive for HPV DNA. Conclusion - No clear correlation exists between HPV genome detection and the histological appearance. HPV-16 genome detection alone may not be a useful predictor of precancerous progression.
引用
收藏
页码:1042 / 1048
页数:7
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