AGE-RELATED-CHANGES IN PROTEOGLYCANS OF HUMAN LIGAMENTUM-FLAVUM

被引：12

作者：

OKADA, A

HARATA, S

TAKEDA, Y

NAKAMURA, T

TAKAGAKI, K

ENDO, M

机构：

[1] Department of Orthopedic Surgery, Hirosaki University School of Medicine, Hirosaki

[2] Department of Biochemistry, Hirosaki University School of Medicine, Hirosaki

来源：

SPINE | 1993年 / 18卷 / 15期

关键词：

LIGAMENTUM-FLAVUM; PROTEOGLYCAN; DERMATAN SULFATE; CHONDROITIN; 6-SULFATE; AGING;

D O I：

10.1097/00007632-199311000-00020

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

摘要：

Age-related changes in proteoglycans of human ligamentum flavum were studied using specimens obtained from patients divided into four age groups. The proteoglycans were purified by ion-exchange and gel-filtration chromatography. A low-molecular-weight proteoglycan with the same molecular size was present in all each age groups. Conversely, high-molecular-weight proteoglycan increased with advancing age. Properties of the sugar chains, glycosaminoglycans, in these proteoglycans were studied using cellulose acetate membrane electrophoresis and chondroitinase digestion. It was found that the high-molecular-weight proteoglycan consisted mainly of chondroitin 6-sulfate, whereas the low-molecular-weight proteoglycan consisted mainly of dermatan sulfate, although the ratio of chondroitin 6-sulfate increased with age. These results indicate that proteoglycans of human ligamentum flavum show changes in amount and composition with age.

引用

页码：2261 / 2266

页数：6

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