EXPRESSION OF HSP70 INDUCED IN CHO CELLS BY 45.0-DEGREES-C HYPERTHERMIA IS CELL-CYCLE ASSOCIATED AND DNA-SYNTHESIS DEPENDENT

被引：20

作者：

HANG, HY

FOX, MH

机构：

[1] COLORADO STATE UNIV,DEPT RADIOL HLTH SCI,FT COLLINS,CO 80523

[2] COLORADO STATE UNIV,GRAD PROGRAM CELL & MOLEC BIOL,FT COLLINS,CO 80523

来源：

CYTOMETRY | 1995年 / 19卷 / 02期

关键词：

HEAT SHOCK PROTEIN; FLOW CYTOMETRY; CELL CYCLE REGULATION;

D O I：

10.1002/cyto.990190206

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In response to a heat shock, the synthesis of heat shock proteins with a molecular weight of 72 kD (hsp72) is induced. To determine whether hsp72 expression in Chinese hamster ovary (CHO) cells is cell cycle associated, now cytometry was used to measure DNA content and heat shock protein levels simultaneously. After 10 min at 45.0 degrees C and incubation at 37 degrees C for several hours, hsp72 was induced to a high level in early S phase. Inhibitors of DNA syn-thesis, aphidicolin, and 1-beta-D-arabinofuranosylcytosine (araC), suppressed the induction of hsp72 but did not inhibit it completely. Hsp73 (constitutive hsp70) was also induced by heat shock, but the induction was uniform through the cell cycle and it was not suppressed by araC. (C) 1995 Wiley-Liss, Inc.

引用

页码：119 / 125

页数：7

共 20 条

[1]

Brown CR, Martin RL, Hansen WJ, Beckmann RP, Welch WJ, The constitutive and stress inducible forms of hsp70 exhibit functional similarities and interact with one another in an ATP‐dependent fashion, J Cell Biol, 5, pp. 1101-1112, (1993)

[2]

Ferrarini M, Heltai S, Zocchi MR, Rugarli C, Unusual expression and localization of heat‐shock proteins in human tumor cells, Int J Cancer, 51, pp. 613-619, (1992)

[3]

Hang H, Fox MH, Low pH suppresses synthesis of HSPs and thermotolerance, Radiat Res, 140, pp. 24-30, (1994)

[4]

Hightower LE, Heat shock, stress proteins, chaperones, and proteotoxicity, Cell, 66, pp. 191-197, (1991)

[5]

Kao HT, Capasso O, Heintz N, Nevins JR, Cell cycle control of the human hsp70 gene: Implications for the role of a cellular E1A‐like function, Mol Cell Biol, 5, pp. 628-633, (1985)

[6]

Milarski KL, Morimoto RI, Expression of human HSP70 during the synthetic phase of the cell cycle, Proc Natl Acad Sci USA, 83, pp. 9517-9521, (1986)

[7]

Milarski KL, Welch WJ, Morimoto RI, Cell cycle‐dependent association of hsp70 with specific cellular proteins, J Cell Biol, 108, pp. 413-423, (1989)

[8]

Morimoto RI, Tissieres A, Georgopoulos C, The stress response function of the proteins, and perspectives, Stress Proteins in Biology and Medicine, pp. 1-36, (1990)

[9]

Nover L, Heat Shock Response of Eukaryotic Cells, (1984)

[10]

Read RA, Fox MH, Bedford JS, The cell cycle dependence of thermotolerance: I. CHO cells heated at 42°C, Radiat Res, 93, pp. 93-106, (1983)

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