LOCATION AND ENCAPSIDATION OF THE COAT PROTEIN CISTRON OF TOBACCO MOSAIC-VIRUS - BIDIRECTIONAL ELONGATION OF THE NUCLEOPROTEIN ROD

The coat protein cistron of tobacco mosaic virus has been located on the viral RNA starting between 975 and 1050 nucleotides from the 3′‐hydroxyl end. This locates its 5′ end close to the origin for virus assembly, where the first protein disk interacts with the RNA. It also means that the coat protein mRNA must have a short 5′‐untranslated tail and a long (over 500 nucleotides) 3′ one. The recovery of characteristic oligonucleotides in nuclease‐protected rods during the growth from RNA and a protein disk preparation shows that elongation of the nucleated rods proceeds independently in both directions though, on average, much more rapidly along the longer 5′ tail than the shorter 3′ tail. protected RNA of length equal to that in the complete virion is first seen within 6 min, showing that the most rapidly elongated particles are substantially complete by this time. Copyright © 1979, Wiley Blackwell. All rights reserved