FUSION OF PROTOPLASTS AND GENETIC-RECOMBINATION OF BREVIBACTERIUM-FLAVUM

被引：77

作者：

KANEKO, H ^{[1
]}

SAKAGUCHI, K ^{[1
]}

机构：

[1] MITSUBISHI KASEI INST LIFE SCI,MICROBIOL CHEM LAB,MACHIDA,TOKYO 194,JAPAN

来源：

AGRICULTURAL AND BIOLOGICAL CHEMISTRY | 1979年 / 43卷 / 05期

关键词：

D O I：

10.1080/00021369.1979.10863583

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Protoplasts of Brevibacterium flavum cells were obtained by treatment with 0.3 units of penicillin per ml during exponential growth, and followed by lysozyme treatment in a hypertonic medium. Protoplasts of antibiotic-resistant and auxotrophic strains were fused by a modified polyethyleneglycol technique, and allowed to revert to bacillary form on selective media. The clones were analyzed and recombinants were found not to segregate. © 1979 Taylor & Francis Group, LLC.

引用

页码：1007 / 1013

页数：7

共 11 条

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