THE CONCENTRATION OF RED-BLOOD-CELL UDPGLUCOSE AND UDPGALACTOSE DETERMINED BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：40

作者：

PALMIERI, MJ ^{[1
]}

BERRY, GT ^{[1
]}

PLAYER, DA ^{[1
]}

ROGERS, S ^{[1
]}

SEGAL, S ^{[1
]}

机构：

[1] UNIV PENN,SCH MED,DEPT PEDIAT,PHILADELPHIA,PA 19104

来源：

ANALYTICAL BIOCHEMISTRY | 1991年 / 194卷 / 02期

关键词：

D O I：

10.1016/0003-2697(91)90246-P

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed a sensitive method that employs high-performance liquid chromatography to separate and quantitate uridine diphosphogalactose (UDPGal) and uridine diphosphoglucose (UDPGlu) in human red blood cells. The trichloracetic acid extracts of red blood cells were chromatographed using a Dionex CarboPac anion-exchange resin and a 20-40% potassium phosphate buffer, pH 4.5, in a gradient-elution program. UDPGal and UDPGlu were detected spectrophotometrically at 254 nm. Recoveries of UDPGal and UDPGlu ranged from 96 to 106%. Under these conditions, there was exceptionally good reproducibility in stored specimens, and the method was sensitive in the low picamole range. The mean values and standard deviations in adults were 2.9 ± 0.4 and 7.8 ± 0.8 μmol/100 g Hgb for UDPGal and UDPGlu, respectively. The values in children were 4.5 ± 1.2 and 10.2 ± 1.7 μmol/100 g Hgb for UDPGal and UDPGlu, respectively. Values determined by the HPLC method are in excellent agreement with those obtained by enzyme analysis. © 1991.

引用

页码：388 / 393

页数：6

共 10 条

[1]

BERGREN WR, 1973, BIOCHIM BIOPHYS ACTA, V315, P464, DOI 10.1016/0005-2744(73)90277-5

[2] GALACTOSE AND GLUCOSE-METABOLISM IN ISOLATED PERFUSED SUCKLING AND ADULT RAT-LIVER [J].