STEREOCHEMICAL ANALYSIS OF BETAMETHASONE AND DEXAMETHASONE BY DERIVATIZATION AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：19

作者：

CHEN, SH ^{[1
]}

WU, SM ^{[1
]}

WU, HL ^{[1
]}

机构：

[1] KAOHSIUNG MED COLL,GRAD INST PHARMACEUT SCI,KAOHSIUNG 807,TAIWAN

来源：

JOURNAL OF CHROMATOGRAPHY | 1992年 / 595卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(92)85163-N

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A simple and economical high-performance liquid chromatographic method has been developed for the simultaneous determination of betamethasone and dexamethasone. The method is based on the derivatization of the structural epimers of betamethasone and dexamethasone with a homochiral reagent, N-carbobenzoxy-L-phenylalanine. The derivatives obtained were easily recognized by a non-chiral silica column with n-hexane dichloromethane-isopropanol (100:100:4, v/v/v) as a mobile phase and a good separation was obtained for quantitation. The method was satisfactorily applied to the determination of betamethasone and dexamethasone in tablets.

引用

页码：203 / 208

页数：6

共 14 条

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