COMBINATION OF POLYMER-BOUND CHARGED GROUPS AND AFFINITY LIGANDS FOR EXTRACTION OF ENZYMES BY PARTITIONING IN AQUEOUS 2-PHASE SYSTEMS

The partition of glucose-6-phosphate dehydrogenase in a water-dextran-polyethylene glycol two-phase system was influenced by the mutual addition of polymers carrying charged groups and affinity ligands. These polymers included DEAE-dextran, dextran sulphate, ligand-dextran, ligand-polyethylene glycol, ligand-Ficoll and ligand-DEAE-dextran, where the ligands used were the dyes Cibacron Blue F3G-A and Procion Yellow HE-3G. Some of these two-phase systems were also used for the counter-current distribution of yeast proteins, demonstrating the separation of several enzymes.