HEPATITIS-C VIRAL CDNA CLONES ISOLATED FROM A HEALTHY CARRIER DONOR IMPLICATED IN POSTTRANSFUSION NON-A, NON-B HEPATITIS

被引：78

作者：

TAKEUCHI, K

BOONMAR, S

KUBO, Y

KATAYAMA, T

HARADA, H

OHBAYASHI, A

CHOO, QL

KUO, G

HOUGHTON, M

SAITO, I

MIYAMURA, T

机构：

[1] NATL INST HLTH,DEPT ENTEROVIRUSES,2-10-35 KAMIOSAKI,SHINAGAWA KU,TOKYO 141,JAPAN

[2] NATL TOKYO CHEST HOSP,DEPT LIVER DIS,KIYOSE,TOKYO 204,JAPAN

[3] CHIRON CORP,EMERYVILLE,CA 94608

[4] NATL TOKYO CHEST HOSP,DEPT SURG,KIYOSE,TOKYO 204,JAPAN

来源：

GENE | 1990年 / 91卷 / 02期

关键词：

antibody assay; Blood transfusion; flaviviridae; polymerase chain reaction; recombinant DNA; sequence homologies;

D O I：

10.1016/0378-1119(90)90102-W

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Using a specific hepatitis C virus (HCV) antibody assay, positive blood donors responsible for the transmission of post-transfusion non-A, non-B hepatitis (PT-NANBH) were identified. cDNA fragments were isolated from one of the plasma samples of such healthy HCV carriers by using polymerase chain reactions. Nucleotide (nt) sequence analyses of the cDNA from three different regions of the viral genome revealed that they were derived from a Japanese HCV isolate that was similar but not identical to the prototype HCV previously isolated from a chronically infected chimpanzee. Homology at the nt and amino acid levels was comparatively lower in the presumed structural region than in putative nonstructural regions. This result not only confirms that HCV antibody-positive contains infectious HCV, but suggests the existence of different type(s) of HCV. © 1990.

引用

页码：287 / 291

页数：5

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