MOLECULAR-CLONING AND NUCLEOTIDE-SEQUENCE DETERMINATION OF THE BACILLUS-STEAROTHERMOPHILUS NCA-1503 SUPEROXIDE-DISMUTASE GENE AND ITS OVEREXPRESSION IN ESCHERICHIA-COLI

被引：6

作者：

BREHM, JK ^{[1
]}

CHAMBERS, SP ^{[1
]}

BROWN, KJ ^{[1
]}

ATKINSON, T ^{[1
]}

MINTON, NP ^{[1
]}

机构：

[1] PUBL HLTH LAB SERV,CTR APPL MICROBIOL & RES,DIV BIOTECHNOL,MOLEC GENET GRP,SALISBURY SP4 0JG,WILTS,ENGLAND

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 1991年 / 36卷 / 03期

关键词：

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The gene (sod) encoding Bacillus stearothermophilus Mn-superoxide dismutase (MnSOD) has been cloned in Escherichia coli and its entire nucleotide sequence determined. With the exception of the post-translationally cleaved N-terminal methionine residue, the predicted amino acid sequence exhibits complete identity to the previously determined amino acid sequence. The recombinant MnSOD was shown to be functionally active in E. coli both in vitro and in vivo, and was expressed to 49% of the soluble cell protein by coupling its transcription to the E. coli trp promoter. The sequenced region of DNA was also found to encompass a second open reading frame. The putative encoded polypeptide exhibited no significant primary sequence homology to any currently characterised protein.

引用

页码：358 / 363

页数：6

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