PROTEINS INTERACTING WITH AN ANDROGEN-RESPONSIVE UNIT IN THE C3(1) GENE INTRON

被引:47
作者
CELIS, L
CLAESSENS, F
PEETERS, B
HEYNS, W
VERHOEVEN, G
ROMBAUTS, W
机构
[1] CATHOLIC UNIV LEUVEN,FAC GENEESKUNDE,AFDELING BIOCHEM,CAMPUS GASTHUISBERG,HERESTR 49,B-3000 LOUVAIN,BELGIUM
[2] CATHOLIC UNIV LEUVEN,FAC GENEESKUNDE,EXPTL GENEESKUNDE & ENDOCRINOL LAB,B-3000 LOUVAIN,BELGIUM
关键词
SEQUENCE-SPECIFIC DNA-BINDING PROTEIN; INTRONIC REGULATORY ELEMENT; PROSTATIC BINDING PROTEIN; ANDROGEN RESPONSE;
D O I
10.1016/0303-7207(93)90165-G
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The expression of the three genes encoding the components Cl, C2 and C3 of prostatic binding protein (PBP) is under androgen control and restricted to the rat ventral prostate. The SstI-PvuII fragment of the first intron of the C3(1) gene displays two binding sites for ubiquitous transcription factors and one for a tissue-specific factor in a 80-bp region upstream of its androgen response element (ARE). The octamer transcription factor 1 (OTF-1) binds to the most distal element (site 1) while a member of the nuclear factor I (NF-1) family recognizes site 2. A third unidentified prostate-specific factor, which also occurs in castrated rats, interacts with the proximal element (site 3). In T-47D cells, both the OTF-1 and the NF-I-like factor can modulate the androgen response of the promoter in a reporter gene construct containing the C3(1) intronic fragment.
引用
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页码:165 / 172
页数:8
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