URINARY-EXCRETION AND ORIGIN OF 11-DEHYDRO-2,3-DINOR-THROMBOXANE-B2 IN MAN

被引：15

作者：

CHIABRANDO, C ^{[1
]}

RIVOLTELLA, L ^{[1
]}

ALBERTI, E ^{[1
]}

BONOLLO, M ^{[1
]}

DJURUP, R ^{[1
]}

FANELLI, R ^{[1
]}

机构：

[1] NOVO NORD,DK-2880 BAGSVAERD,DENMARK

来源：

PROSTAGLANDINS | 1993年 / 45卷 / 05期

关键词：

D O I：

10.1016/0090-6980(93)90117-P

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The in vivo biosynthesis of thromboxane B2 (TXB2) in man is currently evaluated by measuring urinary excretion of its major urinary metabolites, 11-dehydro-TXB2 and 2,3-dinor-TXB2. 11-Dehydro-2,3-dinor-TXB2, another prominent metabolite of exogenous TXB2 in man, has never been measured in human urine. We measured urinary 11-dehydro-2,3-dinor-TXB2 in parallel with 11-dehydro-TXB2 and 2,3-dinor-TXB2 by immunoaffinity extraction/gas chromatography-mass spectrometry in healthy non-smokers (n=12) and age-matched smokers (n=11). In non-smokers, urinary excretion of 11-dehydro-2,3-dinor-TXB2, 11-dehydro-TXB2 and 2,3-dinor-TXB2 was 29.7 +/- 11.1, 53.6 +/- 15.0 and 13.5 +/- 2.8 ng/h (mean +/- SD), respectively. In smokers, only urinary excretion of 2,3-dinor-TXB2 was significantly different (19.7 +/- 6.7 ng/h, p<0.01). Selective inhibition of platelet thromboxane biosynthesis by chronic low-dose aspirin (30 mg/day for 8 days, 4 subjects) comparably reduced platelet-derived metabolites and 11-dehydro-2,3-dinor-TXB2, suggesting that the latter also derives from platelets in healthy subjects.

引用

页码：401 / 411

页数：11

共 15 条

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