RECOGNITION OF B-CELL AND T-CELL EPITOPES BY CATTLE IMMUNIZED WITH A SYNTHETIC PEPTIDE-CONTAINING THE MAJOR IMMUNOGENIC SITE OF VP1 FMDV 01-CAMPOS

被引：30

作者：

ZAMORANO, P ^{[1
]}

WIGDOROVITZ, A ^{[1
]}

CHAHER, MT ^{[1
]}

FERNANDEZ, FM ^{[1
]}

CARRILLO, C ^{[1
]}

MARCOVECCHIO, FE ^{[1
]}

SADIR, AM ^{[1
]}

BORCA, MV ^{[1
]}

机构：

[1] INTA,CICV,INST VIROL,BUENOS AIRES,ARGENTINA

来源：

VIROLOGY | 1994年 / 201卷 / 02期

关键词：

D O I：

10.1006/viro.1994.1305

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The precise location of B and T cell epitopes have been established in a peptide containing the major immunogenic site (residues 135-160) of FMDV strain 01 Campos (01C) VP1. The peptide (p135-160), administered free or conjugated to bovine serum albumin, induced complete protection in guinea pigs and a strong neutralizing antibody (NAb) response in cattle. Using a set of partially overlapping peptides it was shown that although several a cell epitopes were distributed along the p135-160, the residues responsible for the induction of NAb were restricted to the amino acids 135-144. In addition, bovines immunized with the p135-160 showed a strong proliferative response to this peptide but a very poor response against purified virus. Two T-epitopes were located in the 135-160 sequence: one on the region 135-144 and the other in the region 150-160. The immunodominance of these two T cell epitopes was confirmed in cattle immunized with inactivated virus vaccines. (C) 1994 Academic Press, Inc.

引用

页码：383 / 387

页数：5

共 14 条

[1]

BORCA MV, 1993, IMMUNOLOGY, V79, P368

[2] NEW APPROACHES TO VACCINATION AGAINST FOOT-AND-MOUTH-DISEASE [J].