Generation of a murine monoclonal antibody specific for N-glycolylneuraminic acid-containing gangliosides that also recognizes sulfated glycolipids

被引：76

作者：

Vazquez, AM

Alfonso, M

Lanne, B

Karlsson, KA

Carr, A

Barroso, O

Fernandez, LE

Rengifo, E

Lanio, ME

Alvarez, C

Zeuthen, J

Perez, R

机构：

[1] DANISH CANC SOC, DIV CANC BIOL, DEPT TUMOR CELL BIOL, DK-2100 COPENHAGEN, DENMARK

[2] CTR MOLEC IMMUNOL, DEPT RES & DEV, HAVANA 16040, CUBA

[3] GOTHENBURG UNIV, DEPT BIOCHEM MED, S-41390 GOTHENBURG, SWEDEN

[4] CTR MOLEC IMMUNOL, DIV DIAGNOST, HAVANA 11600, CUBA

[5] UNIV HAVANA, FAC BIOL, HAVANA, CUBA

来源：

HYBRIDOMA | 1995年 / 14卷 / 06期

关键词：

D O I：

10.1089/hyb.1995.14.551

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The P3 murine monoclonal antibody (MAb) was generated by immunizing BALB/c mice with NeuGcGM3 included into liposomes. The specificity of this MAb was defined by an enzyme-linked immunosorbent assay and immunostaining on thin-layer chromatograms. P3 MAb binds to NeuGc-containing gangliosides and was shown also to react with sulfated glycolipids. A preliminary immunohistochemical study showed that the P3 MAb was able to recognize antigens expressed in human breast tumors.

引用

页码：551 / 556

页数：6

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