PHARMACOLOGICAL EVIDENCE FOR THE MODULATION OF MONOAMINE RELEASE BY ADENOSINE IN THE INVERTEBRATE NERVOUS-SYSTEM

Abstract: An in vitro preparation from the pedal ganglia of the marine bivalve, Mytilus edulis, was used to examine the modulation of transmitter release by adenosine and its analogs from invertebrate nervous tissue. The ganglia of this organism contain the monoamines dopamine (DA), serotonin (5‐HT), and norepinephrine (NE), and the presynaptic release of these substances is known to be calcium‐dependent. This organism also contains a DA‐sensitive adenylate cyclase system which resembles that seen in mammals. Neural tissue from the pedal ganglia was incubated with labeled monoamines, and release studies were then conducted in superfusion chambers; release of monoamines was evoked by the addition of 50 mM KCl. Addition to the superfusion medium of the adenosine analog, 5′‐N‐ethylcarboxamidoadenosine (NECA; 10 nM), inhibited the release of 5‐HT and DA, and to a lesser extent NE, whereas 100‐fold higher concentrations of adenosine itself and the adenosine analog, R‐N6‐phenylisopropyladenosine, were required to achieve comparable levels of inhibition. The inhibitory effects of NECA on neurotransmitter release were blocked by the adenosine receptor antagonist, theophylline (IC50= 10–14 μM). The results from this study indicate for the first time the possible role of adenosine as a modulator of neurotransmitter release in the invertebrate nervous system. Copyright © 1990, Wiley Blackwell. All rights reserved