LIGAND-EXCHANGE CHROMATOGRAPHY OF FREE AMINO-ACIDS ON PHOSPHATED ZIRCONIUM-OXIDE SUPPORTS

被引：15

作者：

BLACKWELL, JA ^{[1
]}

CARR, PW ^{[1
]}

机构：

[1] UNIV MINNESOTA,INST ADV STUDIES BIOPROC TECHNOL,MINNEAPOLIS,MN 55455

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1992年 / 15卷 / 05期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

D O I：

10.1080/10826079208018831

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Phosphated zirconium oxide particles loaded with copper ions are a mechanically superior alternative to resin based supports for ligand exchange separations of amino acids. They show good stability under acidic and alkaline conditions and afford different selectivity than resin based exchangers. Acidic amino acids are more strongly retained on this phase than on silica or resin based supports due to interactions with the underlying zirconia surface. These interactions occur despite rigorous phosphation of the zirconia particle. The operational aspects of amino acid separations on this material are examined and compared to conventional resin based models.

引用

页码：727 / 751

页数：25

共 22 条

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