ANALYSIS ON HETEROGENEITY OF SQUAMOUS-CELL CARCINOMA ANTIGEN BY 2-DIMENSIONAL ELECTROPHORESIS

被引：19

作者：

ABE, H

OKUNO, N

TAKEDA, O

SUMINAMI, Y

KATO, H

NAKAMURA, K

机构：

[1] YAMAGUCHI UNIV, SCH MED, DEPT OBSTET & GYNECOL, UBE, YAMAGUCHI 755, JAPAN

[2] YAMAGUCHI UNIV, SCH MED, DEPT BIOCHEM 1, UBE, YAMAGUCHI 755, JAPAN

来源：

ELECTROPHORESIS | 1994年 / 15卷 / 07期

关键词：

D O I：

10.1002/elps.11501501145

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Squamous cell carcinoma (SCC) antigen was separated by two-dimensional electrophoresis combined with immunoblotting into four spots: spot 1 with pI 6.4 and 44.5 kDa, spot 2 with pI 6.3 and 44.5 kDa, spot 3 with pI 6.0 and 44.5 kDa, and spot 4 with pI 5.9 and 45 kDa. In cancer and noncanerous tissues, it was common that spot 1 was the largest spot. In noncancerous tissues, spot 3 was the smallest spot and spot 2 was stained as densely as spot 4. In cancer tissues, however, spot 4 was apparently smaller than spot 2 and 3. Also, spots 2 and 3 in cancer tissues were larger than those in noncancerous tissues. When SCC antigen was treated with alkaline phosphatase prior to isoelectric focusing (IEF), spot 4 disappeared from the immunoblotting pattern. When the SCC antigen was treated with alkaline phosphatase after IEF, spot 4 changed its molecular weight to the same weight as that of the other three spots. These results strongly suggest that spot 4 is phosphorylated SCC antigen.

引用

页码：988 / 991

页数：4

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