THE LIGHT RESPONSE OF DROSOPHILA PHOTORECEPTORS IS ACCOMPANIED BY AN INCREASE IN CELLULAR CALCIUM - EFFECTS OF SPECIFIC MUTATIONS

被引：120

作者：

PERETZ, A

SUSSTOBY, E

ROMGLAS, A

ARNON, A

PAYNE, R

MINKE, B

机构：

[1] HEBREW UNIV JERUSALEM,HADASSAH MED SCH,MINERVA CTR STUDIES VISUAL TRANSDUCT,IL-91120 JERUSALEM,ISRAEL

[2] UNIV MARYLAND,DEPT ZOOL,COLL PK,MD 20742

来源：

NEURON | 1994年 / 12卷 / 06期

关键词：

D O I：

10.1016/0896-6273(94)90442-1

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Photoreceptors of dissociated Drosophila retinae were loaded with the fluorescent Ca2+ indicators, fluo-3 and Calcium Green-5N. In fluo-3-loaded, wild-type photoreceptors, a rapid increase in fluorescence (Ca2+ signal) accompanied the light-evoked inward current. Removal of extracellular Ca2+ greatly reduced the Ca2+ signal, indicating Ca2+ influx as its major cause. In Calcium Green-SN-loaded trp mutants, which lack a large fraction of the Ca2+ permeability underlying the light-evoked inward current, the Ca2+ signal was smaller relative to wild-type photoreceptors. Fluo-3-loaded norpA mutant photoreceptors, which lack a light-activated phospholipase C, generated no light-evoked inward current and no Ca2+ signal. The phosphoinositide pathway therefore appears necessary for both excitation and changes in cytosolic free Ca2+ concentration.

引用

页码：1257 / 1267

页数：11

共 49 条

[1] CALCIUM-SENSITIVITY AND INOSITOL POLYPHOSPHATE-SENSITIVITY OF THE CALCIUM-SEQUESTERING ENDOPLASMIC-RETICULUM IN THE PHOTORECEPTOR CELLS OF THE HONEYBEE DRONE [J].