TRANSCRIPTIONAL REGULATION OF THE MTS1 GENE IN HUMAN LYMPHOMA-CELLS - THE ROLE OF DNA-METHYLATION

被引：41

作者：

TULCHINSKY, E ^{[1
]}

GRIGORIAN, M ^{[1
]}

TKATCH, T ^{[1
]}

GEORGIEV, G ^{[1
]}

LUKANIDIN, E ^{[1
]}

机构：

[1] INST GENE BIOL,MOSCOW,RUSSIA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1995年 / 1261卷 / 02期

关键词：

MTS1; GENE; DNA METHYLATION; DNAASEI HYPERSENSITIVITY SITE; GENE TRANSCRIPTION;

D O I：

10.1016/0167-4781(95)00013-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The transcription of the mts1 gene putatively involved in the control of tumor metastasis was studied in three human lymphoma cell lines: MOLT-4, CEM and Jurkat. The level of the mts1 gene transcription is high in MOLT-4 cells, lower in CEM cells and hardly detectable in Jurkat cells. This correlates with the hypomethylation of DNA in the first exon and the first intron of the mts1 gene in the analyzed culture cells. This area was also found to be undermethylated in human peripheral blood cells - macrophages, neutrophils and lymphocytes where the mts1 gene is highly expressed. 5-Azadeoxycytidine (AzadC) - an inhibitor of the eukaryotic DNA-methylase significantly induces the expression of the mts1 gene in CEM and Jurkat cells and has little effect on mts1 gene transcription in MOLT-4 cells. The drug does not influence mts1 transcription in cultivated peripheral blood lymphocytes. These data indicate the possible involvement of the methylation of the first exon/first intron sequences in the transcriptional repression of the mts1 gene. The finding of two DNAaseI hypersensitivity sites (DHSs) mapped in the first intron of the mts1 gene supports this suggestion.

引用

页码：243 / 248

页数：6

共 23 条

[11]

Dorin, Emslie, Van Heyningen, Genomics, 8, pp. 420-426, (1990)

[12]

Tulchinsky, Ford, Kramerov, Reshetnyak, Grigorian, Zain, Lukanidin, Proc. Natl. Acad. Sci. USA, 89, pp. 9146-9150, (1992)

[13]

Tulchinsky, Kramerov, Ford, Reshetnyak, Lukanidin, Zain, Oncogene, 8, pp. 79-86, (1993)

[14]

Sambrook, Fritsch, Mariatis, Molecular Cloning: A Laboratory Manual, (1989)

[15]

Yeivin, Razin, DNA methylation, pp. 523-569, (1993)

[16]

Antequera, Boyes, Bird, Cell, 62, pp. 503-514, (1990)

[17]

Keshet, Lieman-Hurwitz, Cedar, Cell, 44, pp. 535-543, (1986)

[18]

Jones, Cell, 40, pp. 485-486, (1985)

[19]

Weih, Nitsch, Reik, Schutz, Becker, EMBO J., 10, pp. 2559-2567, (1991)

[20]

Fajkus, Vyskot, Bezdek, FEBS Lett., 314, pp. 13-16, (1992)

← 1 2 3 →