ENZYMATIC SYNTHESIS OF OLIGOGUANYLIC ACIDS CONTAINING 2'-5'-PHOSPHODIESTER LINKAGES

Under certain conditions Tl ribonuclease catalyzes the synthesis of (2′-5′) GpGp from G-cyclic-p. The evidence for this is that GpG (obtained from the GpGp) yields (2′) Gp when specifically degraded by periodate oxidation followed by amine catalyzed β elimination of the 5′-linked nucleoside. The T1 synthesized GpG has an ORD distinctly different from (3′-5′) GpG and is resistant to Tl hydrolysis. © 1969.