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2-DIMENSIONAL CRYSTALLIZATION OF DNA GYRASE-B SUBUNIT ON SPECIFICALLY DESIGNED LIPID MONOLAYERS

被引：37
作者：
LEBEAU, L
REGNIER, E
SCHULTZ, P
WANG, JC
MIOSKOWSKI, C
OUDET, P
机构：
[1] FAC MED STRASBOURG,CNRS,GENET MOLEC EUCARYOTES LAB,F-67085 STRASBOURG,FRANCE
[2] HARVARD UNIV,DEPT BIOCHEM & MOLEC BIOL,CAMBRIDGE,MA 02138
来源：
FEBS LETTERS | 1990年 / 267卷 / 01期
关键词：
DNA gyrase; Electron microscopy: Image processing; Novobiocin; Protein-lipid interaction; Two-dimensional crystallization;
D O I：
10.1016/0014-5793(90)80282-N
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The B subunit of DNA gyrase formed two-dimensional crystals when bound to a specifically recognized phospholipid spread into a monolayer at the air/water interface. The especially designed lipids consisted of novobiocin coupled through the 3' or 2″ hydroxyl group and a hydrophilous linker of a given length to dioleoylphosphatidic acid. Two-dimensional crystals of the gyrase B subunit are formed under physiological conditions of pH and ionic strength, with no precipitant added to the solution. Crystal diffraction extended to a 2.7 nm resolution in negative stain, with unit cell parameters a = 6.1 nm, b = 7.6 nm and γ = 64°. © 1990.
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页码：38 / 42
页数：5
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