REVERSED-PHASE LIQUID-CHROMATOGRAPHY OF POLAR BENZENE-DERIVATIVES ON POLY(VINYLBENZO-18-CROWN-6)-IMMOBILIZED SILICA AS A STATIONARY PHASE

A packing in which poly(vinylbenzo-18-crown-6) is immobilized covalently to silica was tested for its usefulness as a stationary phase in the reversed-phase liquid chromatography of polar disubstituted benzene derivatives. Both electrostatic and hydrophobic interactions contribute considerably to the chromatographic separation. When potassium cations, which are complexed by the crown ether moiety on the stationary phase, are added to the mobile phase during chromatography using the poly(crown ether)-immobilized silica, positive charges are generated on the surface of the stationary phase and some electrostatic interaction occurs between polar disubstituted benzene derivatives and the stationary phase. Hence the retention behaviour of polar organic compound can be controlled easily by adding metal cations to the mobile phase. This type of reversed-phase chromatography using poly(crown ether)-immobilized silica is useful for separating positional isomers of various disubstituted benzene derivatives. © 1990.