CRYSTALLIZATION OF THE FV FRAGMENT OF MOUSE MYELOMA PROTEIN-M315

被引：9

作者：

ASCHAFFENBURG, R ^{[1
]}

PHILLIPS, DC ^{[1
]}

ROSE, DR ^{[1
]}

SUTTON, BJ ^{[1
]}

DOWER, SK ^{[1
]}

DWEK, RA ^{[1
]}

机构：

[1] UNIV OXFORD,DEPT BIOCHEM,OXFORD OX1 3QU,ENGLAND

来源：

BIOCHEMICAL JOURNAL | 1979年 / 181卷 / 02期

关键词：

D O I：

10.1042/bj1810497

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Fv fragment of mouse myeloma protein M315 was crystallized from poly(ethylene glycol) solution in the form of monoclinic crystals, space group C2 and unit cell dimensions a=5.96 nm (59.6 Å), b=5.66 nm (56.6 Å), c=13.79 nm (137.9 Å) and β=99.7°. Some unusual effects of poly(ethylene glycol) on protein crystals were noted and are discussed.

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页码：497 / 499

页数：3

相关论文

共 19 条

[1]

DUGAN ES, 1973, BIOCHEMISTRY-US, V12, P5400

[2] STRUCTURE OF AN ANTIBODY COMBINING SITE BY MAGNETIC-RESONANCE [J].

DWEK, RA ;

WAINHOBSON, S ;

DOWER, S ;

GETTINS, P ;

SUTTON, B ;

PERKINS, SJ ;

GIVOL, D .

NATURE, 1977, 266 (5597) :31-37

[3] MOUSE MYELOMA PROTEINS WITH ANTIHAPTEN ANTIBODY ACTIVITY . PROTEIN PRODUCED BY PLASMA CELL TUMOR MOPC-315 [J].

EISEN, HN ;

SIMMS, ES ;

POTTER, M .

BIOCHEMISTRY, 1968, 7 (11) :4126-&

[4] AMINO-ACID SEQUENCE OF VARIABLE REGION OF HEAVY (ALPHA) CHAIN OF A MOUSE MYELOMA PROTEIN WITH ANTIHAPTEN ACTIVITY [J].

FRANCIS, SH ;

LESLIE, RGQ ;

HOOD, L ;

EISEN, HN .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1974, 71 (04) :1123-1127

[5] AFFINITY LABELING OF A MOUSE MYELOMA PROTEIN WHICH BINDS NITROPHENYL LIGANDS - KINETICS OF LABELING AND ISOLATION OF A LABELED PEPTIDE [J].

GOETZL, EJ ;

METZGER, H .

BIOCHEMISTRY, 1970, 9 (05) :1267-&

[6] ACTIVE ANTIBODY FRAGMENT (FV) COMPOSED OF VARIABLE PORTIONS OF HEAVY AND LIGHT CHAINS [J].

HOCHMAN, J ;

INBAR, D ;

GIVOL, D .

BIOCHEMISTRY, 1973, 12 (06) :1130-1135

[7]

IMOTO T, 1972, ENZYMES, V7, P665

[8]

INBAR D, 1971, J BIOL CHEM, V246, P6272

[9] LOCALIZATION OF ANTIBODY-COMBINING SITES WITHIN VARIABLE PORTIONS OF HEAVY AND LIGHT CHAINS [J].

INBAR, D ;

GIVOL, D ;

HOCHMAN, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1972, 69 (09) :2659-&

[10]

Kabat E A, 1978, Adv Protein Chem, V32, P1, DOI 10.1016/S0065-3233(08)60574-4