INVIVO METABOLISM OF FLUORESCENT CERAMIDE IN CENTRAL-NERVOUS-SYSTEM MYELIN OF ADULT-RATS

被引：6

作者：

DIBIASE, A ^{[1
]}

ARGIOLAS, L ^{[1
]}

CONFALONI, A ^{[1
]}

SALVATI, S ^{[1
]}

机构：

[1] IST SUPER SANITA,DEPT PHARMACOL,I-00161 ROME,ITALY

来源：

NEUROCHEMICAL RESEARCH | 1991年 / 16卷 / 05期

关键词：

CNS; MYELIN; CERAMIDE; SPHINGOLIPIDS; INVIVO METABOLISM;

D O I：

10.1007/BF00974873

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The metabolism of sphingolipids in the central nervous system (CNS) has been studied in adult rats by intraventricular administration of fluorescent ceramide (CER). Rats were sacrificed at various time points post inoculation and the fluorescence of CER, cerebrosides (CB), sulfatides (SULF) and sphingomyelin (SPM) was determined in the CNS myelin and in the pellet, containing mainly microsomes, obtained by Norton myelin preparation. The incorporation of fluorescence was more in the pellet than in the myelin at all times studied. Initially the fluorescence present in the pellet was prevalently due to untransformed CER but an increase of fluorescent products with time was observed. CB was the main product up to 2 h post inoculation, then it decreased with concomitant increase of fluorescent SULF. In the myelin we did not observe differences in incorporation and transformation of fluorescent CER with time: CB was the main fluorescent product at all times studied. At 0.5 h post inoculation the fluorescence, observed by fluorescence microscope, was located in the cell lining the ventricles while after 24 h it appeared also in the paraventricular areas.

引用

页码：551 / 554

页数：4

共 19 条

[1]

BURKART T, 1982, J BIOL CHEM, V257, P3151

[2]

DAVISON AN, 1970, COMPR BIOCHEM PHYSIO, V18, P293

[3]

FOLCH J, 1957, J BIOL CHEM, V226, P497

[4]

GRUBER H, 1981, TECHNIQUES NEUROANAT, P14