NOVEL ROLE OF HUMAN CHORIONIC-GONADOTROPIN IN DIFFERENTIATION OF HUMAN CYTOTROPHOBLASTS

被引:232
作者
SHI, QJ [1 ]
LEI, ZM [1 ]
RAO, CV [1 ]
LIN, J [1 ]
机构
[1] UNIV LOUISVILLE, SCH MED, DEPT OBSTET & GYNECOL, 438 MDR BLDG, LOUISVILLE, KY 40292 USA
关键词
D O I
10.1210/en.132.3.1387
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Our laboratory previously demonstrated that cytotrophoblasts and syncytiotrophoblasts in human placental tissue contain hCG/LH receptors. From this finding, we postulated that one role of hCG might be to promote the differentiation of cytotrophoblasts into syncytiotrophoblasts. To test this postulate, cytotrophoblasts were isolated from human term pregnancy placentas and cultured with and without increasing concentrations of highly purified hCG. The results showed that hCG had a biphasic effect on 1) the aggregation of cells without intervening plasma membranes; 2) the expression of cadherin, a cell adhesion receptor that facilitates cellular aggregation; 3) the expression of hCG/LH receptor gene; and 4) the expression of three different hormonal markers of differentiation. The hCG effects were time and dose dependent and hormone specific. The addition of excess polyclonal hCG antibody, but not normal rabbit serum or nonspecific antirabbit immunoglobulin G, decreased basal responses as well as those to exogenous hCG. The polyclonal hCG/LH receptor antibody increased differentiation and dramatically stimulated hCG secretion in the presence or absence of exogenous hCG. (Bu)2CAMP mimicked the actions of hCG. H-89, a protein kinase-A inhibitor, decreased basal as well as exogenous hCG responses. Calphostin, a protein kinase-C inhibitor and lavendustin, a tyrosine kinase inhibitor, on the other hand, bad no effect. In summary, it is novel that hCG made in human placenta can regulate the differentiation of cytotrophoblasts, which make little hCG, into syncytiotrophoblasts, which make considerable amounts of hCG.
引用
收藏
页码:1387 / 1395
页数:9
相关论文
共 47 条
[1]  
ALSAT E, 1974, CR ACAD SCI D NAT, V278, P2665
[2]   IMMUNOHISTOCHEMICAL LOCALIZATION OF FOLLICLE-STIMULATING-HORMONE, LUTEINIZING-HORMONE, GROWTH-HORMONE, ADRENOCORTICOTROPIC HORMONE AND PROLACTIN IN THE HUMAN-PLACENTA [J].
ALTIMIMI, A ;
FOX, H .
PLACENTA, 1986, 7 (02) :163-172
[3]   AGGREGATION OF DISPERSED HUMAN CYTOTROPHOBLASTIC CELLS - LESSONS RELEVANT TO THE MORPHOGENESIS OF THE PLACENTA [J].
BABALOLA, GO ;
COUTIFARIS, C ;
SOTO, EA ;
KLIMAN, HJ ;
SHUMAN, H ;
STRAUSS, JF .
DEVELOPMENTAL BIOLOGY, 1990, 137 (01) :100-108
[4]  
Boyd JD, 1970, HUMAN PLACENTA
[5]  
CASPO AI, 1972, AM J OBSTET GYNECOL, V112, P1061
[6]   INFLUENCE OF LUTEINIZING HORMONE ON AROMATIZATION OF TESTOSTERONE BY HUMAN PLACENTA PERFUSED IN VITRO [J].
CEDARD, L ;
ALSAT, E ;
EGO, C ;
VARANGOT, J .
STEROIDS, 1968, 11 (02) :179-&
[7]   STUDIES ON MODE OF ACTION OF LUTEINIZING HORMONE AND CHORIONIC GONADOTROPIN ON ESTROGENIC BIOSYNTHESIS AND GLYCOGENOLYSIS BY HUMAN PLACENTA PERFUSED IN-VITRO [J].
CEDARD, L ;
ALSAT, E ;
URTASUN, MJ ;
VARANGOT, J .
STEROIDS, 1970, 16 (04) :361-&
[8]  
DANIELSMCQUEEN S, 1987, TROPHOBLAST RES, V2, P423
[9]  
DAVIS JS, 1984, J BIOL CHEM, V259, P5028
[10]  
DEMERS LM, 1973, BIOCHIM BIOPHYS ACTA, V313, P202