PRELIMINARY CRYSTALLOGRAPHIC STUDIES OF THE LIGAND-BINDING DOMAIN OF THE THYROID-HORMONE RECEPTOR COMPLEXED WITH TRIIODOTHYRONINE

A truncated, recombinant form of the thyroid hormone receptor, including the hormone binding domain, has been co-crystallized with the hormone T3. The crystals are monoclinic, most likely space group P2, with two molecules per asymmetric unit and cell dimensions a = 63·6 Å, b =80·8 Å, c = 100·9 Å and β = 92·1 °. The crystals diffract to only medium resolution and decay rapidly in the X-ray beam using laboratory sources. By contrast, high resolution, high-quality data are obtained using synchrotron radiation in conjunction with cryocrystallography. © 1994 Academic Press, Inc.