REGIONS OF EVOLUTIONARY CONSERVATION BETWEEN THE RAT AND HUMAN PROHIBITIN-ENCODING GENES

被引：24

作者：

ALTUS, MS

WOOD, CM

STEWART, DA

ROSKAMS, AJI

FRIEDMAN, V

HENDERSON, T

OWENS, GA

DANNER, DB

JUPE, ER

DELLORCO, RT

MCCLUNG, JK

机构：

[1] OKLAHOMA MED RES FDN,NOBLE CTR BIOMED RES,OKLAHOMA CITY,OK 73104

[2] NIA,GENET MOLEC LAB,BALTIMORE,MD 21224

来源：

GENE | 1995年 / 158卷 / 02期

关键词：

PROMOTER; TRANSCRIPTION INITIATION; INTRON; SPLICE JUNCTION; ANTIPROLIFERATIVE; TUMOR SUPPRESSOR;

D O I：

10.1016/0378-1119(95)00164-2

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have analyzed and compared the 5' promoter region, the intron structure and the exon-intron flanking sequences in the rat and human prohibitin-encoding genes (PHB). Comparative analysis of a 350-nt region immediately 5' to and including the first exon identifies eight highly conserved regions, four of which correspond to binding sites for known transcriptional control proteins (CCAAT box, 'SV40' site and two Sp1 sites). The promoter lacks a TATA box. Four transcription start points (tsp) clustered within a 35-bp region were identified by rapid amplification of cDNA ends (RACE). The exon-intron boundaries in rat and human are highly conserved, with identical positioning of splice junctions. PCR analysis with conserved exon primers was used to detect length variation between rat and human PHB, and length differences were observed in all of the introns.

引用

页码：291 / 294

页数：4

共 24 条

[1] Black, Solomon, The search for the familial breast/ovarian cancer gene, Trends Genet., 9, pp. 22-26, (1993)
[2] Black, Nicolai, Borrow, Solomon, Somatic cell hybrid map of the long arm of human chromosome 17, containing the familial breast cancer locus (BRCA 1), Am. J. Hum. Genet., 52, pp. 702-710, (1993)
[3] Bohmann, Keller, Dale, Scholer, Tebb, Mattaj, A transcription factor which binds to the enhancers of SV40, immunoglobulin heavy chain and U2 snRNA genes, Nature, 325, pp. 268-272, (1987)
[4] Bryant, Towards the cellular functions of tumor suppresors, Trends Cell Biol., 3, pp. 31-35, (1993)
[5] Chodosh, Baldwin, Carthew, Sharp, Human CCAAT-binding proteins have heterologous subunits, Cell, 53, pp. 11-24, (1988)
[6] Chodosh, Olesen, Hahn, Baldwin, Guarente, Sharp, A yeast and a human CCAAT-binding protein have heterologous subunits that are functionally interchangeable, Cell, 53, pp. 25-35, (1988)
[7] Davidson, Fromental, Augereau, Wilderman, Zenke, Chambon, Cell-type specific protein binding to the enhancer of simian virus 40 in nuclear extracts, Nature, 323, pp. 544-548, (1986)
[8] Eveleth, Marsh, Sequence and expression of the Cc gene, a member of the dopa decarboxylase gene cluster of Drosophila
[9] possible translational regulation, Nucleic Acids Res., 14, pp. 6169-6183, (1986)
[10] Franklin, Jazwinski, A yeast homolog of the rat prohibitin gene is differentially expressed and determines longevity in Saccharomyces cerevisiae, J. Cell. Biochem. Suppl., 17 D, (1993)

← 1 2 3 →