GENETIC STABILITY OF COLD-ADAPTED A/LENINGRAD/134/47/57 (H2N2) INFLUENZA-VIRUS - SEQUENCE-ANALYSIS OF LIVE COLD-ADAPTED REASSORTANT VACCINE STRAINS BEFORE AND AFTER REPLICATION IN CHILDREN

被引：24

作者：

KLIMOV, AI

EGOROV, AY

GUSHCHINA, MI

MEDVEDEVA, TE

GAMBLE, WC

RUDENKO, LG

ALEXANDROVA, GI

COX, NJ

机构：

[1] CTR DIS CONTROL & PREVENT,NATL CTR INFECT DIS,DIV VIRAL & RICKETTSIAL DIS,INFLUENZA BRANCH G16,ATLANTA,GA 30333

[2] RUSSIAN ACAD MED SCI,VIRAL PREPARAT RES INST,MOSCOW 109088,RUSSIA

[3] RUSSIAN ACAD MED SCI,EXPTL MED RES INST,ST PETERSBURG 197022,RUSSIA

来源：

JOURNAL OF GENERAL VIROLOGY | 1995年 / 76卷

关键词：

D O I：

10.1099/0022-1317-76-6-1521

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We previously reported that the A/Leningrad/134/47/57 (H2N2) cold-adapted virus (A/Len/47) used in preparing reassortant live attenuated vaccines for children acquired 14 (11 coding) mutations in genes coding for proteins other than haemagglutinin and neuraminidase during cold-adaptation. Preservation of these mutations in genomes of viruses isolated from children on the second, fifth, or eighth day after vaccination was examined by sequence analysis. The sequence data demonstrated that all nine coding mutations selected for examination were conserved in the genomes of all 11 strains investigated, indicating that the mutations accompanying cold-adaptation and attenuation of the A/Len/47 master vaccine are highly stable.

引用

页码：1521 / 1525

页数：5

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