CAPILLARY GAS-LIQUID-CHROMATOGRAPHIC SEPARATION OF OXO AND OXO-HYDROXY BILE-ACID ISOMERS OF THE ALLO AND NORMAL SERIES

Capillary gas-liquid chromatographic separation was studied for 60 oxo and oxo-hydroxy bile acids of the allo (5α-H, A/B-trans) and normal (5β-H, A/B-cis) series, which differ from one another in the number, position, and configuration of oxo and hydroxy groups at positions C-3, C-4, C-6, C-7, and/or C-12. Good separation of the isomers was achieved by the use of an aluminum-clad fused-silica capillary column coated with bonded and cross-linked methyl polysiloxane. © 1985 American Oil Chemists' Society.