DETERMINATION OF BENZALKONIUM CHLORIDE IN AQUEOUS OPHTHALMIC PREPARATIONS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：43

作者：

GOMEZGOMAR, A

GONZALEZAUBERT, MM

GARCESTORRENTS, J

COSTASEGARRA, J

机构：

[1] Analytical Research Department, Laboratorios Cusí, S.A., Barcelona

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 1990年 / 8卷 / 8-12期

关键词：

BENZALKONIUM CHLORIDE; OPHTHALMIC PRESERVATIVES; BENZALKONIUM HOMOLOGS; CYANO-PROPYL COLUMN; TRIETHYLAMINE-ELUENT MODIFIER; HPLC;

D O I：

10.1016/0731-7085(90)80135-C

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A high-performance liquid chromatographic method is described for the determination of homologues of benzalkonium chloride in aqueous ophthalmic preparations. The technique involves direct injection of the sample on a 5-mu-m Spherisorb-CN column. The mobile phase is acetonitrile-triethylamine (0.1%, v/v) in water (pH 2.5; 40:60, v/v). Detection is carried out at 215 nm. The method is rapid, specific reproducible and simple, and is especially useful for the assay of this preservative in stability studies and quality control procedures.

引用

页码：871 / 876

页数：6

共 7 条

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