PURIFICATION AND PARTIAL CHARACTERIZATION OF MILK CLOTTING PROTEASES FROM FLOWERS OF CYNARA-CARDUNCULUS

被引:160
作者
HEIMGARTNER, U
PIETRZAK, M
GEERTSEN, R
BRODELIUS, P
FIGUEIREDO, ACD
PAIS, MSS
机构
[1] SWISS FED INST TECHNOL,INST BIOTECHNOL,CH-8093 ZURICH,SWITZERLAND
[2] FAC CIENCIAS LISBOA,DEPT BIOL VEGETAL,P-1700 LISBON,PORTUGAL
关键词
Compositae; Cynara cardunculus; dried flowers; enzyme purification; milk clotting; proteases;
D O I
10.1016/0031-9422(90)80090-4
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Three proteases (cynarases 1, 2 and 3) with milk-clotting activity have been purified from dried flowers of Cynara cardunculus. The proteases are each composed of one large and one small subunit. The native Mr of the dimeric proteins is 49 000. The three proteases are glycoproteins containing N-linked high mannose type glycans. Cynarase 3 shows the highest proteolytic and milk-clotting activity. All three enzymes express maximum activity at pH 5.1. Inhibitor studies indicate that the cynarases are of the aspartic acid type. Antibodies raised against the large subunit of cynarase 3 cross-reacts with the large subunits of the other two cynarases after destruction of the glycan structure by periodate oxidation. © 1990.
引用
收藏
页码:1405 / 1410
页数:6
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