FORMATION OF SPHEROIDS OF ADULT-RAT HEPATOCYTES ON POLYLYSINE-COATED SURFACES AND THEIR ALBUMIN PRODUCTION

被引：17

作者：

SAKAI, Y

SUZUKI, M

机构：

[1] Institute of Industrial Science, University of Tokyo, Tokyo, 106, 7-22-1 Roppongi, Minato-ku

来源：

BIOTECHNOLOGY TECHNIQUES | 1991年 / 5卷 / 04期

关键词：

D O I：

10.1007/BF02438667

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Adult rat hepatocytes in primary culture formed spheroidal aggregates on the surfaces precoated with more than 0.01 mg-polylysine/mL. Epidermal growth factor (EGF) improved the formation and the maintenance of spheroid. The albumin secretion of the spheroids formed was maintained about 2 weeks at a high level.

引用

收藏

页码：299 / 302

页数：4

相关论文

共 8 条

[1] ASSAY FOR NANOGRAM QUANTITIES OF DNA IN CELLULAR HOMOGENATES [J].

BRUNK, CF ;

JONES, KC ;

JAMES, TW .

ANALYTICAL BIOCHEMISTRY, 1979, 92 (02) :497-500

[2] HEPATOCYTE FUNCTION AND EXTRACELLULAR-MATRIX GEOMETRY - LONG-TERM CULTURE IN A SANDWICH CONFIGURATION [J].

DUNN, JCY ;

YARMUSH, ML ;

KOEBE, HG ;

TOMPKINS, RG .

FASEB JOURNAL, 1989, 3 (02) :174-177

[3] HEPATOCYTE PROLIFERATION INVITRO - ITS DEPENDENCE ON THE USE OF SERUM-FREE HORMONALLY DEFINED MEDIUM AND SUBSTRATA OF EXTRACELLULAR-MATRIX [J].

ENAT, R ;

JEFFERSON, DM ;

RUIZOPAZO, N ;

GATMAITAN, Z ;

LEINWAND, LA ;

REID, LM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (05) :1411-1415

[4] MAINTENANCE AND REVERSIBILITY OF ACTIVE ALBUMIN SECRETION BY ADULT-RAT HEPATOCYTES CO-CULTURED WITH ANOTHER LIVER EPITHELIAL-CELL TYPE [J].

GUGUENGUILLOUZO, C ;

CLEMENT, B ;

BAFFET, G ;

BEAUMONT, C ;

MORELCHANY, E ;

GLAISE, D ;

GUILLOUZO, A .

EXPERIMENTAL CELL RESEARCH, 1983, 143 (01) :47-54

[5] CONTINUED HIGH ALBUMIN PRODUCTION BY MULTICELLULAR SPHEROIDS OF ADULT-RAT HEPATOCYTES FORMED IN THE PRESENCE OF LIVER-DERIVED PROTEOGLYCANS [J].

KOIDE, N ;

SHINJI, T ;

TANABE, T ;

ASANO, K ;

KAWAGUCHI, M ;

SAKAGUCHI, K ;

KOIDE, Y ;

MORI, M ;

TSUJI, T .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 161 (01) :385-391

[6] FORMATION OF MULTICELLULAR SPHEROIDS COMPOSED OF ADULT-RAT HEPATOCYTES IN DISHES WITH POSITIVELY CHARGED SURFACES AND UNDER OTHER NONADHERENT ENVIRONMENTS [J].

KOIDE, N ;

SAKAGUCHI, K ;

KOIDE, Y ;

ASANO, K ;

KAWAGUCHI, M ;

MATSUSHIMA, H ;

TAKENAMI, T ;

SHINJI, T ;

MORI, M ;

TSUJI, T .

EXPERIMENTAL CELL RESEARCH, 1990, 186 (02) :227-235

[7] SPHEROIDAL AGGREGATE CULTURE OF RAT-LIVER CELLS - HISTOTYPIC REORGANIZATION, BIOMATRIX DEPOSITION, AND MAINTENANCE OF FUNCTIONAL ACTIVITIES [J].

LANDRY, J ;

BERNIER, D ;

OUELLET, C ;

GOYETTE, R ;

MARCEAU, N .

JOURNAL OF CELL BIOLOGY, 1985, 101 (03) :914-923

[8]

Seglen P O, 1976, Methods Cell Biol, V13, P29, DOI 10.1016/S0091-679X(08)61797-5