EMBEDDING FREE-FLOATING CELLS AND MICROSCOPIC PARTICLES - SERUM ALBUMIN COAGULUM - EPOXY RESIN

被引:40
作者
SHANDS, JW
机构
[1] Department of Microbiology, University of Florida, College of Medicine, Gainesville, FL
来源
STAIN TECHNOLOGY | 1968年 / 43卷 / 01期
关键词
D O I
10.3109/10520296809115036
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Small quantities of cells or microscopic particles are fixed in suspension, centrifuged at low speed in a conical tube and the supernatant fluid removed as completely as possible. The pellet is resuspended in 0.25 ml of 2% bovine serum albumin (in 0.05 M Tris buffer, pH 7.5) and transferred to a small, conical cellulose tube (Beckman #303369). One drop of 25% glutaraldehyde is mixed with the suspension and the tube is quickly centrifuged in a swinging bucket rotor. A gel forms in about 5 min and the coagulum can be cut and processed through embedding like small blocks of tissue. © 1968 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.
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页码:15 / &
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