MOLECULAR-CLONING OF SHEEP T-CELL RECEPTOR GAMMA-CHAIN AND DELTA-CHAIN CONSTANT REGIONS - UNUSUAL PRIMARY STRUCTURE OF GAMMA-CHAIN HINGE SEGMENTS

被引:34
作者
HEIN, WR
DUDLER, L
MARCUZ, A
GROSSBERGER, D
机构
[1] Basel Institute for Immunology, Basel
[2] Department of Cell Biology, Stanford University, Stanford, California
关键词
D O I
10.1002/eji.1830200826
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The primary structure of sheep T cell receptor (TcR) γ and δ chain constant (C) regions has been determined by cDNA cloning. A comparison of the nucleotide and deduced amino acid sequences of the sheep chains with known human and mouse sequences shows that the primary structure of the immunoglobulin, transmembrane and cytoplasmic Cγ domains and all of the Cδ region has been substantially conserved. However, the hinge or connector region of sheep γ chains differs significantly from all known TcR chains. Clones representing two different sheep Cγ genes were isolated and both contain additional sequence in this region, making them the longest TcR chains so far identified. The hinge region of both sheep Cγ sequences contains two additional cysteine residues and a motif of five amino acids (TTESP or TTEPP) which has been triplicated in one of the clones. Other repetitive segments of 13–17 amino acids could also be identified suggesting that, as in the human Cγ2 gene, this region of the sheep genes could have arisen from an exon duplication or triplication event. Southern blot analysis of sheep DNA confirmed the presence of one Cδ gene and at least two Cγgenes. A restriction fragment length polymorphism that is probably associated with allelic sequence variation in the sheep Cδ gene was detected in DNA from different animals. Although the essential structure of the γ/δ TcR appears well conserved through evolution, the marked heterogeneity evident in the hinge region of γ chains both within and between species, and particularly the presence of additional cysteine residues in the sheep sequences, may be of structural and functional importance. Copyright © 1990 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim
引用
收藏
页码:1795 / 1804
页数:10
相关论文
共 32 条
[1]   LIMITED DIVERSITY OF GAMMA-DELTA-ANTIGEN RECEPTOR GENES OF THY-1+ DENDRITIC EPIDERMAL-CELLS [J].
ASARNOW, DM ;
KUZIEL, WA ;
BONYHADI, M ;
TIGELAAR, RE ;
TUCKER, PW ;
ALLISON, JP .
CELL, 1988, 55 (05) :837-847
[2]  
BRENNER MB, 1988, ADV IMMUNOL, V43, P133
[3]   EXON DUPLICATION AND TRIPLICATION IN THE HUMAN T-CELL RECEPTOR GAMMA CONSTANT REGION GENES AND RFLP IN FRENCH, LEBANESE, TUNISIAN, AND BLACK-AFRICAN POPULATIONS [J].
BURESI, C ;
GHANEM, N ;
HUCK, S ;
LEFRANC, G ;
LEFRANC, MP .
IMMUNOGENETICS, 1989, 29 (03) :161-172
[4]   SUPERCOIL SEQUENCING - A FAST AND SIMPLE METHOD FOR SEQUENCING PLASMID DNA [J].
CHEN, EY ;
SEEBURG, PH .
DNA-A JOURNAL OF MOLECULAR & CELLULAR BIOLOGY, 1985, 4 (02) :165-170
[5]   A NEW T-CELL RECEPTOR GENE LOCATED WITHIN THE ALPHA-LOCUS AND EXPRESSED EARLY IN T-CELL DIFFERENTIATION [J].
CHIEN, YH ;
IWASHIMA, M ;
KAPLAN, KB ;
ELLIOTT, JF ;
DAVIS, MM .
NATURE, 1987, 327 (6124) :677-682
[6]  
ELLIOTT JF, 1988, NATURE, V331, P627
[7]   DIVERSITY, REARRANGEMENT, AND EXPRESSION OF MURINE T-CELL GAMMA-GENES [J].
GARMAN, RD ;
DOHERTY, PJ ;
RAULET, DH .
CELL, 1986, 45 (05) :733-742
[8]  
GROSSBERGER D, 1990, IN PRESS IMMUNOGENET
[9]   ORIGIN OF THY-1+ DENDRITIC EPIDERMAL-CELLS OF ADULT MICE FROM FETAL THYMIC PRECURSORS [J].
HAVRAN, WL ;
ALLISON, JP .
NATURE, 1990, 344 (6261) :68-70
[10]   STRUCTURE, ORGANIZATION, AND SOMATIC REARRANGEMENT OF T-CELL GAMMA-GENES [J].
HAYDAY, AC ;
SAITO, H ;
GILLIES, SD ;
KRANZ, DM ;
TANIGAWA, G ;
EISEN, HN ;
TONEGAWA, S .
CELL, 1985, 40 (02) :259-269