IMPROVED DETECTION OF PERSISTENT FOOT-AND-MOUTH-DISEASE INFECTION IN CATTLE BY THE POLYMERASE CHAIN-REACTION

被引：25

作者：

DONN, A ^{[1
]}

MARTIN, LA ^{[1
]}

DONALDSON, AI ^{[1
]}

机构：

[1] AFRC,INST ANIM HLTH,PIRBRIGHT LAB,WOKING GU24 0NF,SURREY,ENGLAND

来源：

JOURNAL OF VIROLOGICAL METHODS | 1994年 / 49卷 / 02期

关键词：

FOOT-AND-MOUTH DISEASE; CARRIER; PERSISTENCE; POLYMERASE CHAIN REACTION; VIRUS;

D O I：

10.1016/0166-0934(94)90042-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Persistent foot-and-mouth disease (FMD) infection of cattle was established by exposure to infected pigs. Oesophageal-pharyngeal fluid samples collected ante-mortem and tissue samples obtained post-mortem were tested for virus by conventional means and for viral RNA by the polymerase chain reaction (PCR). The results showed that the PCR was superior for detecting the carrier state in tissue samples. A high neutralising activity in tissues probably influenced the yields of virus obtained by infectivity assay.

引用

页码：179 / 186

页数：8

共 22 条

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