THE OCCURRENCE OF A NEW GENETIC VARIANT OF FUSARIUM-OXYSPORUM FSP PISI

被引:14
作者
BODKER, L [1 ]
LEWIS, BG [1 ]
CODDINGTON, A [1 ]
机构
[1] UNIV E ANGLIA,SCH BIOL SCI,NORWICH NR4 7TJ,NORFOLK,ENGLAND
关键词
D O I
10.1111/j.1365-3059.1993.tb02668.x
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
During a survey of root diseases of pea in Denmark, a new genetic variant of Fusarium oxysporum f.sp. pisi was isolated from vining peas in two widely separated geographical regions. In terms of pathogenicity on a set of differential pea lines, the Danish isolated closely resembled a race 6 isolate from the United States. DNA extracts of the isolates: restricted with the endonuclease HindIII, then probed with a homologous repetitive genomic fragment from the plasmid pDG106 by the Southern hybridization technique, gave a unique 'fingerprint' pattern distinctly different from the American race 6 and all other known races. When probed with pDG312, containing a homologous ribosomal repeat unit, the pattern obtained for the Danish isolates-was indistinguishable from races 1, 5 and 6 but distinctly different from 2A and 2B. The Danish isolates represent a separate vegetative compatibility group because they are compatible with each other but incompatible with the other known races. In pigmentation the new variant resembled races 1, 5 and 6 for the first 8-12 days, after which it began to secrete a dark purple pigment resembling that of race 2A and 2B. Until an additional line in the host differentials can separate the new genetic variant it should be considered a subgroup of F. oxysporum f. sp. pisi race 6.
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页码:833 / 838
页数:6
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