FATTY ACYLATION OF 2 INTERNAL LYSINE RESIDUES REQUIRED FOR THE TOXIC ACTIVITY OF ESCHERICHIA-COLI HEMOLYSIN

被引:173
作者
STANLEY, P
PACKMAN, LC
KORONAKIS, V
HUGHES, C
机构
[1] UNIV CAMBRIDGE, DEPT PATHOL, CAMBRIDGE CB2 1QP, ENGLAND
[2] UNIV CAMBRIDGE, DEPT BIOCHEM, CAMBRIDGE CB2 1QW, ENGLAND
关键词
D O I
10.1126/science.7801126
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Hemolysin of Escherichia coli is activated by fatty acylation of the protoxin, directed by the putative acyl transferase HlyC and by acyl carrier protein (ACP). Mass spectrometry and Edman degradation of proteolytic products from mature toxin activated in vitro with tritium-labeled acylACP revealed two fatty-acylated internal lysine residues, lysine 564 and lysine 690. Resistance of the acylation to chemical treatments suggested that fatty acid was amide linked. Substitution of the two lysines confirmed that they were the only sites of acylation and showed that although each was acylated in the absence of the other, both sites were required for in vivo toxin activity.
引用
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页码:1992 / 1996
页数:5
相关论文
共 34 条
[1]   CLONING AND CHARACTERIZATION OF A HEMOLYSIN GENE FROM ACTINOBACILLUS (HEMOPHILUS) PLEUROPNEUMONIAE [J].
CHANG, YF ;
YOUNG, RY ;
STRUCK, DK .
DNA-A JOURNAL OF MOLECULAR & CELLULAR BIOLOGY, 1989, 8 (09) :635-647
[2]   NUCLEOTIDE-SEQUENCE OF AN ESCHERICHIA-COLI CHROMOSOMAL HEMOLYSIN [J].
FELMLEE, T ;
PELLETT, S ;
WELCH, RA .
JOURNAL OF BACTERIOLOGY, 1985, 163 (01) :94-105
[3]   IDENTIFICATION OF RTX TOXIN TARGET-CELL SPECIFICITY DOMAINS BY USE OF HYBRID GENES [J].
FORESTIER, C ;
WELCH, RA .
INFECTION AND IMMUNITY, 1991, 59 (11) :4212-4220
[4]   NONRECIPROCAL COMPLEMENTATION OF THE HLYC AND LKTC GENES OF THE ESCHERICHIA-COLI HEMOLYSIN AND PASTEURELLA-HAEMOLYTICA LEUKOTOXIN DETERMINANTS [J].
FORESTIER, C ;
WELCH, RA .
INFECTION AND IMMUNITY, 1990, 58 (03) :828-832
[5]   NUCLEOTIDE-SEQUENCE OF THE HEMOLYSIN-I GENE FROM ACTINOBACILLUS-PLEUROPNEUMONIAE [J].
FREY, J ;
MEIER, R ;
GYGI, D ;
NICOLET, J .
INFECTION AND IMMUNITY, 1991, 59 (09) :3026-3032
[6]   SECRETION OF CYCLOLYSIN, THE CALMODULIN-SENSITIVE ADENYLATE-CYCLASE HEMOLYSIN BIFUNCTIONAL PROTEIN OF BORDETELLA-PERTUSSIS [J].
GLASER, P ;
SAKAMOTO, H ;
BELLALOU, J ;
ULLMANN, A ;
DANCHIN, A .
EMBO JOURNAL, 1988, 7 (12) :3997-4004
[7]   ISOLATION OF THE ACTINOBACILLUS-PLEUROPNEUMONIAE HEMOLYSIN GENE AND THE ACTIVATION AND SECRETION OF THE PROHAEMOLYSIN BY THE HLYC, HLYB AND HLYD PROTEINS OF ESCHERICHIA-COLI [J].
GYGI, D ;
NICOLET, J ;
FREY, J ;
CROSS, M ;
KORONAKIS, V ;
HUGHES, C .
MOLECULAR MICROBIOLOGY, 1990, 4 (01) :123-128
[8]   INTERNAL LYSINE PALMITOYLATION IN ADENYLATE-CYCLASE TOXIN FROM BORDETELLA-PERTUSSIS [J].
HACKETT, M ;
GUO, L ;
SHABANOWITZ, J ;
HUNT, DF ;
HEWLETT, EL .
SCIENCE, 1994, 266 (5184) :433-435
[9]   INVITRO ACTIVATION OF ESCHERICHIA-COLI PROHAEMOLYSIN TO THE MATURE MEMBRANE-TARGETED TOXIN REQUIRES HLYC AND A LOW-MOLECULAR-WEIGHT CYTOSOLIC POLYPEPTIDE [J].
HARDIE, KR ;
ISSARTEL, JP ;
KORONAKIS, E ;
HUGHES, C ;
KORONAKIS, V .
MOLECULAR MICROBIOLOGY, 1991, 5 (07) :1669-1679
[10]  
HEDO JA, 1987, J BIOL CHEM, V262, P954