RAPID DETECTION OF VIRUS GENOME FROM IMPORTED DENGUE FEVER AND DENGUE HEMORRHAGIC-FEVER PATIENTS BY DIRECT POLYMERASE CHAIN-REACTION

被引：31

作者：

MORITA, K

MAEMOTO, T

HONDA, S

ONISHI, K

MURATA, M

TANAKA, M

IGARASHI, A

机构：

[1] ASAHI CHUO HOSP, DEPT PEDIAT, CHIBA, JAPAN

[2] BOKUTO METROPOLITAN HOSP, DEPT COMMUNICABLE DIS, TOKYO, JAPAN

来源：

JOURNAL OF MEDICAL VIROLOGY | 1994年 / 44卷 / 01期

关键词：

VIRUS DIAGNOSIS; DIRECT RT-PCR; FLAVIVIRUS;

D O I：

10.1002/jmv.1890440111

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Serum specimens from patients with imported dengue fever and dengue hemorrhagic fever were directly subjected to reverse transcription and polymerase chain reaction (RT-PCR) without any RNA purification. The amplified virus genome was detected within 3 hours. The results of PCR corresponded closely to the results of virus isolation using cultured mosquito cells, suggesting that direct RT-PCR procedure greatly facilitates rapid diagnosis of dengue infection. (C) 1994 Wiley-Liss, Inc.

引用

页码：54 / 58

页数：5

共 28 条

[1]

BLACKBURN NK, 1987, S AFR MED J, V71, P386

[2] ANTIBODY-CAPTURE ELISA FOR DETECTION OF IMMUNOGLOBULIN-M ANTIBODIES IN SERA FROM JAPANESE ENCEPHALITIS AND DENGUE HEMORRHAGIC-FEVER PATIENTS [J].