EGG-YOLK RIBOFLAVIN BINDING-PROTEIN AS A NEW CHIRAL STATIONARY-PHASE IN HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：35

作者：

MASSOLINI, G ^{[1
]}

DELORENZI, E ^{[1
]}

PONCI, MC ^{[1
]}

GANDINI, C ^{[1
]}

CACCIALANZA, G ^{[1
]}

MONACO, HL ^{[1
]}

机构：

[1] UNIV PAVIA,DEPT GENET,I-27100 PAVIA,ITALY

来源：

JOURNAL OF CHROMATOGRAPHY A | 1995年 / 704卷 / 01期

关键词：

D O I：

10.1016/0021-9673(95)00043-M

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A chiral stationary phase for high-performance liquid chromatography based on hen egg yolk riboflavin binding protein is introduced. The purified protein was immobilized on activated 5NH(2) Nucleosil silica. Chiral acidic, basic and uncharged drugs were chromatographed and the influence of the mobile phase parameters on the retention times and enantioselectivity was studied. Thirteen out of the twenty compounds tested were partially or baseline resolved. These encouraging preliminary results suggest that this chiral stationary phase may be applicable to a wide range of drug enantiomers in the reversed-phase mode.

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页码：55 / 65

页数：11

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