TN5099, A XYLE PROMOTER PROBE TRANSPOSON FOR STREPTOMYCES SPP

被引:29
作者
HAHN, DR [1 ]
SOLENBERG, PJ [1 ]
BALTZ, RH [1 ]
机构
[1] ELI LILLY & CO,LILLY RES LAB,INDIANAPOLIS,IN 46285
关键词
D O I
10.1128/jb.173.17.5573-5577.1991
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Tn5099, a promoter probe transposon for Streptomyces spp., was constructed by inserting a promoterless xylE gene and a hygromycin resistance gene into IS493. Tn5099 transposed into different sites in the Streptomyces griseofuscus genome, and the xylE reporter gene was expressed in some of the transposition mutants. Strains containing Tn5099 insertions that gave regulated expression of the xylE gene were identified.
引用
收藏
页码:5573 / 5577
页数:5
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