PHOSPHOLIPID TRANSPHOSPHATIDYLATION CATALYZED BY CABBAGE PHOSPHOLIPASE-D IN EMULSION SYSTEMS

Phospholipid modification catalysed by phospholipase D, partially purified from cabbage, has been performed in water-organic solvent emulsions. Phosphatidylcholine reacted with alcohols through nucleophilic substitution of the choline moiety (transphosphatidylation) to form new phospholipids or by hydrolysis, to form phosphatidic acid. The reactions were carried out in two systems based on aqueous buffer and either chloroform or diethyl ether. Products focused on were phosphatidylglycerol, in a preparative-scale reaction, and phosphatidylbutanediol for the study of reaction rates. N-(2-Hydroxyethyl)octanamide affected the product distribution by reducing the rate of hydrolysis more than the rate of transphosphatidylation. phatidylation.