QUANTITATIVE EXTRACTION AND DETERMINATION OF ELEMENTAL SULFUR AND STOICHIOMETRIC OXIDATION OF SULFIDE TO ELEMENTAL SULFUR BY THIOBACILLUS THIOOXIDANS

被引：27

作者：

CHAN, CW ^{[1
]}

SUZUKI, I ^{[1
]}

机构：

[1] UNIV MANITOBA,DEPT MICROBIOL,WINNIPEG R3T 2N2,MB,CANADA

来源：

CANADIAN JOURNAL OF MICROBIOLOGY | 1993年 / 39卷 / 12期

关键词：

ELEMENTAL SULFUR; QUANTITATIVE ANALYSIS; SULFIDE OXIDATION; THIOBACILLUS THIOOXIDANS;

D O I：

10.1139/m93-176

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A sensitive and quantitative analytical method for determining elemental sulfur in a biological system was developed. Elemental sulfur was determined after extraction with petroleum ether by cyanolysis and ferric thiocyanate color formation in acetone. The method was successfully applied to show that sulfide was oxidized by Thiobacillus thiooxidans to elemental sulfur nearly stoichiometrically when further oxidation of elemental sulfur was inhibited by N-ethylmaleimide.

引用

页码：1166 / 1168

页数：3

共 9 条

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