ONE-POT SELECTIVE DEUTERIATION OF 5'-DIMETHOXYTRITYLATED DEOXYNUCLEOTIDE DERIVATIVES

被引：3

作者：

CHEN, ST

CHEN, SY

CHOU, SH

CHEN, CR

HUANG, WC

WANG, KT

机构：

[1] ACAD SINICA,INST BIOL CHEM,POB 23-106,TAIPEI 10098,TAIWAN

[2] NATL TAIWAN NORMAL UNIV,DEPT CHEM,TAIPEI 117,TAIWAN

[3] NATL TAIWAN UNIV,DEPT CHEM,TAIPEI,TAIWAN

[4] UNIV WASHINGTON,HOWARD HUGHES MED INST SL-15,SEATTLE,WA 98195

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 1994年 / 4卷 / 06期

关键词：

D O I：

10.1016/S0960-894X(01)80849-0

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Procedures have been developed for selective incorporation of deuterium at C-6 of 5'-DMT-dC(Bz)@, C-8 of 5'-DMT-dA(Bz), C-8 of 5'-DMT-dG(i-Bu), and C-6 of 5'-DMT-dT in dimethylsulfoxide solution catalyzed by various amounts of base at 90-135-degrees-C with quantitative or nearly quantitative deuterium-labeling yields. The reactions of deuterium exchange of C-6 of 5'-DMT-dC(Bz), C-8 of 5'-DMT-dA(Bz), and C-8 of 5'-DMT-dG(i-Bu) were performed in a one-pot reaction procedure and that of C-6 of 5'-DMT-dT was achieved by deuteriation of 5'-unprotected dT, followed by dimethoxytritylation of 5'-OH with 4,4'-dimethoxytrityl chloride.

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页码：789 / 794

页数：6

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