MOLECULAR-CLONING AND FUNCTIONAL EXPRESSION OF A POTASSIUM CHANNEL CDNA ISOLATED FROM A RAT CARDIAC LIBRARY

被引：137

作者：

TSENGCRANK, JCL

TSENG, GN

SCHWARTZ, A

TANOUYE, MA

机构：

[1] CALTECH,DIV BIOL,PASADENA,CA 91125

[2] COLUMBIA UNIV COLL PHYS & SURG,DEPT PHARMACOL,NEW YORK,NY 10032

来源：

FEBS LETTERS | 1990年 / 268卷 / 01期

关键词：

Cardiac tissue expression; PCR cloning; Transient outward K[!sup]+[!/sup] current; Xenopus oocyte expression;

D O I：

10.1016/0014-5793(90)80973-M

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A full-length K+ channel cDNA (RHK1) was isolated from a rat cardiac library using the polymerase chain reaction (PCR) method and degenerate oligonucleotide primers derived from K+ channel sequences conserved between Drosophila Shaker H4 and mouse brain MBK1. Although RHK1 was isolated from heart, its expression was found in both heart and brain. The RHK1-encoded protein, when expressed in Xenopus oocytes, gated a 4-aminopyridine (4-AP)-sensitive transient outward current. This current is similar to the transient outward current measured in rat ventricular myocytes with respect to voltage-dependence of activation and inactivation, time course of activation and inactivation, and pharmacology. © 1990.

引用

页码：63 / 68

页数：6

共 27 条

[1]

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